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To address these issues, the current protocol employs the DTNB reagent to measure GPx activity without protein precipitation, effectively solving all previous problems
A GST mutant in which four glutamine residues were replaced with asparagine (GST 4QN ) exhibited a glutathione binding affinity similar to that of wild-type GST and could be purified by glutathione affinity chromatography
Protocol: Dilute Glutathione 2400mg with 20ml NaCl 0.9%, Add Glutathione and Vit C 10g/20ml & Thioctic Acid 300mg/12ml , Tranexamic Acid 250mg to 500ml NaCl 0.9% bag and administer
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