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5 HiScript II qRT SuperMix (Reverse transcription reagent) and TRIzol were obtained from Vazyme (Nanjing, China)

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In acute iron exposure, Nrf2 may protect from iron-induced injury, whereas long-term iron exposure resulted in iron accumulation, cytosolic ROS formation, and increased HO-1 (HMOX-1) mRNA expression, and this was accompanied by nuclear translocation of Nrf2 and induction of its target protein NQO1 (van Raaij et al., 2018)

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