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The NIH Office of Dietary Supplements, citing Institute of Medicine recommendations, advises that it is advisable for those older than 50 years to meet their RDA mainly by consuming foods fortified with vitamin B12 or a supplement containing vitamin B12. This recommendation reflects the fact that 10 to 30% of older adults lose the ability to efficiently absorb naturally occurring B12 from food
The sodium citrate-buffered saline was removed using an Amicon Ultra-4 ultrafiltration tube with a molecular weight cut-off of 10,000 Da (Millipore, Bedford, MA, USA) at 4500 g and 4 C for 45 min
The absence of preservatives ensures purity, but it also limits reuse
Nutrients, 14 (17), 3629
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