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Qi SN, Zhang ZF, Wang ZY, Yoshida A, Ueda T
The deltoid and gluteal muscles are common sites for these injections
The erythrocytes were then lysed with lysis buffer (BD Pharm Lyse, BD Bioscience, San Jose, USA) for 10 min followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and resuspension of the cells in Hepes-buffered saline (HBS, 14 mM Hepes, 0.9% NaCl)
Patients may need 100500 mg/day IV thiamine to prevent or sometime leads Wernickes encephalopathy a life-threatening complication causing confusion, memory loss, and poor coordination
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