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Agar gels were mixed with 1 mL PBS by vigorous pipetting before the addition of calcein-AM, DCFDA, or mBcl to the same concentration as indicated for cells cultured in liquid medium and incubated for 30 min in the dark at the temperature indicated for those cells

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Since dopamine has been associated with locomotor deficits, it can be speculated that its regulatory role on dopamine might have contributed to the improvement in locomotor activity