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A critical barrier to effective therapy is the bloodbrain barrier (BBB), a selective interface that restricts the entry of most therapeutic agents into the brain [3, 4]
For the inhibition assay, the concentrations of substrates in the cell lysate treated with different concentrations of inhibitors were determined via LC-MS, and the calculation methods for the uptake rate and UR were similar to those in the substrate assay
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doi: 10.1016/S1472-6483(10)61174-1
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