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Western blot analysis RIPA Lysis Buffer (TransGen, Beijing, China) was used to extract total protein from tissues/fibroblasts and a Nuclear and Cytoplasmic Protein Extraction Kit (TransGen) was used to extract the protein from nucleus and cytoplasm of fibroblasts

### Step 6: Let It Dissolve Set the vial down and wait 1-2 minutes

Antibiotics, particularly tetracyclines, anticonvulsants like phenobarbital, and certain chemotherapy agents may interact negatively with the B vitamins