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Experimental groups consisted of 20 animals each, and the overall design of the study is presented in Figure 1
On the latter, studies conducted using site-specific brain micro-injections and single-photon emission computed tomography documented a novel regulatory role of the hypothalamic supramammillary nucleus in the regulation of food intake and reward, posing this brain area as one of the main targets of the GLP1/E conjugate, along with the lateral hypothalamus and the nucleus of the solitary tract, whose activation may be responsible for the anorectic, weight-lowering and reward reducing effects of this multi-agonist 51
Results of these were compared with the level of antioxidant enzymes in mice following administration of crude C
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