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Briefly, for mammalian-expressed PV VLPs, PV-specific gene sequences for the P1 region were codon optimised for expression in mammalian cells and cloned into modified vaccinia virus Ankara (MVA) transfer vectors upstream of an uncleavable 3CD sequence derived from PV1 Mahoney with native sequence

Stackhouse S, Miller PL, Park SK, Meyer TW

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Above 8C, thermal energy disrupts the bonds faster than they can reform