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In cases of significant nausea, pharmacologic interventions such as prochlorperazine may be used as needed and are preferred over ondansetron
Robotic setup: robotic arms are connected to the instruments placed through those incisions
After incubation (10 min, 39C), the plates were centrifuged (200 x g, 5 min, 20C, Universal 320, Hettich, Tuttlingen, Germany) and the medium was replaced with pre-warmed XF Seahorse medium supplemented with glucose, sodium pyruvate and glutamine (all purchased from Agilent, Santa Clara, California, USA)
This would be the exception, not the rule
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