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10.1016/j.freeradbiomed.2014.07.019 46 PittsM.HoffmannP
After 2h shaking at 30C, cells were harvested and underwent two centrifugation steps at 1,500 g for 3 min at room temperature, followed by resuspension in isosmotic buffer to a final concentration of 1.5 OD600 units/ml
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