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Patients should seek immediate medical attention for signs of possible complications such as cellulitis (redness, warmth, fever), deep vein thrombosis (sudden unilateral swelling, calf pain), or significant worsening of symptoms
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The outcome incident or recurrent diagnosis of CUD (International Classification of Diseases, Tenth Revision (ICD-10) code F12 Cannabis related disorders) that occurred within the 12-month time window after the index event) were compared between matched cohorts
After overnight culture, the cells were co-transfected with pGloSensor22F cAMP plasmid (Promega) and GLP-1R DNA using Lipofectamine 2000 transfection reagent (Invitrogen)
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