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Quantitative RT-PCR assays were performed using the QuantStudio 3 Real-Time PCR system (Applied Biosystems) with the TB Green Fast qPCR Mix (Takara, RR430) or the PowerUp SYBR Green Master Mix for qPCR (Thermo Fisher Scientific)

Moreover, the recent advances in applications of nano-lipid carriers for treating different skin diseases are summarized

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These innovative regenerative therapies promise not only symptom suppression , but also the reversal of the auditory and neural injury at the heart of tinnitus, offering long-term solutions where conventional medicine has failed [6-10]