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Other brain regions that exhibited considerable GLP-1R expression included the ventral pallidum, anterior pretectal nucleus, pontine nuclei, dentate gyrus, and lateral reticular nucleus (Supplemental Figure 1A)

second-generation long-acting analogues such as cagrilintide under late-stage clinical development, often combined with GLP-1 therapy for enhanced weight loss), (b) small molecules or peptides designed to inhibit amylin misfolding and aggregation to protect -cells, and (c) immunotherapeutic or vaccine approaches targeting pathological IAPP species

Clinical data across multiple GLP-1 trials consistently shows that 25 to 40% of total weight lost comes from lean tissue

Briefly, FLS were seeded on glass slides, washed twice with D-Hanks, fixed with 4% paraformaldehyde, and then permeabilized with 0.2% Triton X-100