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At day 6, medium was changed into neuronal maintenance medium consisting of: Neurobasal (Gibco TM , Thermo Fisher Scientific, Netherlands #21103049) containing 1:50 B-27 supplement without vitamin A (Gibco TM , Thermo Fisher Scientific, Netherlands #12587010) and 1:100 GlutaMax (Gibco TM , Thermo Fisher Scientific, Netherlands #35050061)
If symptoms of hypothyroidism appear or lab values change, stop the supplement and consult your clinician
Guedes de Sa, K
Beyond that distinction, general handling principles gentle introduction of bacteriostatic water, swirling rather than shaking, and prompt refrigerated storage apply the same way to blended vials as they do to single-peptide vials
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