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RNA extraction and qRT-PCR Total RNA was extracted from 20 freshly dissected pairs of male testes for each replicate using TRIzol (Ambion) and quantified by spectrophotometric analysis (Nanodrop, Thermo Scientific)
BPC-157: A synthetic 15-amino acid peptide structurally derived from a protein naturally found in gastric secretions
doi: 10.1016/j.cell.2012.03.042 18 Brigelius-FloheRMaiorinoM
Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a puncture-proof sharps container afterward [14]
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