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The trial protocol used a stepwise escalation schedule, and researchers noted lower discontinuation rates with slower titration
This leads to: More glucose remaining in circulation after meals More insulin release to compensate More energy stored as fat initially subcutaneous, but eventually as visceral fat As VAT accumulates, it drives inflammation, insulin resistance, and metabolic dysfunction creating a vicious cycle
The medium was refreshed daily throughout the 5-day differentiation period
The Lancet, Volume 402, Issue 10403, 720730
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