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Population Pharmacokinetic Modelling Datasets containing plasma concentration and covariate data from each trial were converted to a format compatible with the nonlinear mixed-effects modelling (NONMEM) software (ICON Development Solutions, Ellicott City, MD, USA), version 7.1.2

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Notably, GSH binding motif is not conserved among the other seleno-GPxs (GPx2, GPx3, GPx4, and GPx6) justifying their abilities to use wide variety of thiols like cysteine, protein thiols, mercaptoethanol, and dithiothreitol in addition to GSH as reducing equivalent for the catalysis [8, 17, 18]

In total, more than 300 constituents have been identified in propolis samples, whereas the biological activity of propolis has been mainly attributed to a handful of substances, including flavonoids, terpenes, caffeic acid, ferulic acid, cumaric acid and esters