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For batch fermentation, pre-cultured cells were harvested and inoculated into main cultures to an initial optical density at 600 nm (OD 600 ) of 1.0

The same volume of Cytotox-ONE Reagent (Promega, USA) was added into each well and the microplate was shaken for 30 secs to mix

Intermittent fasting works by extending the overnight fasting window, typically eating within an 8-hour window and fasting for 16 hours, or using a 5:2 approach with two very low calorie days per week

Given the high cost of GLP-1s, the patients in that arm of the study may also have had greater access to resources (exercise programs, health coaches, and nutritionists), she says