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Samples were centrifuged with a swing-out rotor to pass cells through the density cushion (25 min, 2,750 g , 4 C and acceleration and deceleration set to 40% of maximum)

In laboratory research settings, GHK-Cu has been investigated for its effects on fibroblast gene expression profiles, collagen and glycosaminoglycan synthesis characterization in cell culture models, and antioxidant enzyme upregulation via Nrf2 pathway activation