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After the membranes were incubated in blocking buffer at room temperature for 2 h, they were washed 3 times in tris-buffered saline and tween 20 (TBST) solution, 5 min each, and then incubated with primary antibodies overnight at 4 C
Although the specific molecular mechanisms through which obesity promotes carcinogenesis remain not fully elucidated, epidemiological studies indicate that approximately 48% of malignant tumors can be attributed to obesity, and obesity serves as an independent risk factor for poor prognosis in cancer patients [54]
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