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Cells were cultured in Dulbeccos Modified Eagles medium (Welgene LM001-05) supplemented with 10% fetal bovine serum (Gibco 16000-044), and 1% penicillin/streptomycin (Gibco 15140-122)
The advantage is that measurement precision is very forgiving at this concentration
Again, folic acid deficiency may lower the assayable levels of B12 while increasing homocysteine levels
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