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Methods Animals We used 6-week-old male C57BL/6J mice (obtained from the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology) and 6-week-old male Nrf2 knockout mice (B6.129 1-Nfe2l2 tm1Ywk /J, purchased from Jackson Laboratory, Bar Harbor, ME) for the experiments

Follow the units on the syringe if .5mL (50 unit syringe) or 1mL (100 unit syringe) Inject once per week, DO NOT inject daily

Compounded Semaglutide is a customized version of the medication Semaglutide, which is commonly used to manage Type 2 diabetes and promote weight loss

This is most relevant for weight-based peptides like kisspeptin (dosed in mcg/kg) and less relevant for receptor-saturating peptides like BPC-157, where tissue concentration at the injury site matters more than total body distribution