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Center for Digestive and Liver Diseases, Nara City Hospital, Nara, Japan Sapienza University of Rome, Unit of Rheumatology, Rome, Italy Enrico Arosio, MD, Sergio De Marchi, MD and others

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doi: 10.1186/s13054-025-05516-2 58

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For intracellular staining of phospho(p)-mTOR (Fisher Scientific 15549836) and p-S6 (eBioscience 15528216), cells were first stained for extracellular markers, fixed in 2% FA at RT, and stained for p-mTOR and p-S6 diluted 1:200 in saponin 5 g/mL

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