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For the HEDS assays, either GSH was varied between 100 M and 1.5 mM for ScGrx (or 250 M and 3.0 mM for PfGrx) at fixed concentrations of HEDS (0.18, 0.37, 0.55 and 0.74 mM) or HEDS was varied between 90 and 920 M at fixed concentrations of GSH (0.3, 0.5, 1 and 1.5 mM for ScGrx7 or 0.25, 0.5, 1 and 2 mM for PfGrx)
No published human trial has tested all three together
While some foods contain natural amounts of CoQ10, for your hearts support external sources of CoQ10 are available
Results were reported as 490 nm values subtracted from the 680 values, averaged, three biological replicates were repeated per cell line, and graphs were generated using GraphPad Prism (version 10.3.1), reporting mean LDH activity SD
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