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Whilst the stability of V 1 seemed unaffected, label-free proteomics of ACT-treated and untreated ATP6AP1 KO cells displayed decreased steady-state levels of all detected V 0 subunits, most prominently of the ATP6AP1-associated heterodimer-forming subunit ATP6AP2 (Fig
Centrifuge the homogenized tissue sample at 8,000 g for 10 min
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