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Avoid repeated freeze-thaw cycles, which degrade peptide structural integrity
I know that this is not anything of concern. For Gal, the benefit of knowing and the cost of monitoring outweighs the risk of false positives
Then 80% of the supernatant was taken and dried down before reconstitution in 100 l of mobile phase A (90:5:5 ACN:H 2 O:100 mM ammonium formate (AmFm) in H 2 O) and vortexed for ~10 s
This will help us understand why it might cause certain side effects
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