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(8) Matthew Tucker, Addison Keely, Joo Young Park, Manuela Rosenfeld, et al (2023) Intranasal GHK peptide enhances resilience to cognitive decline in aging mice bioRxiv [Preprint], 2023 Nov 17:2023.11.16.567423

Once O 3 contacts body fluids and tissues it rapidly reacts with all macromolecules (lipids, proteins, carbohydrates, and DNA) of the cellular membranes [15] to create hydrogen peroxide (ROS-reactive oxygen species) and a mixture of lipid ozonation products (LOP), mainly composed of 4-hydroxynonenal (4-HNE), one of the major end products of lipid peroxidation and an inducer of oxidative stress, and trans-4 hydroxy-2-hexenal (4-HHE) from polyunsaturated fatty acids (PUFA)

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On the other hand, Th17 cells and IL-17 present in the skin activate a nearby inflammatory response mediated by neutrophils, which ultimately results in damage to the tissue (122, 123)