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Immunoblot assays Cell lysates from HCC-1806, PC3 WT and PC3 GGT1 + cells were obtained by placing PBS-washed cell plates on ice and scraping with RIPA buffer (Thermo Scientific, 89900) containing Halt Protease and Phosphatase Inhibitor cocktail (Thermo Scientific, 1861280)
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Expression analysis of recombinant lysyl oxidase (LOX) in myofibroblastlike cells
The strongest review found 36 studies, but 35 were preclinical and only 1 was clinical, with no large human efficacy trials identified
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