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However, bacterial biofilm in chronic wounds may cause irreversible bacterial adhesion, leading to persistent inflammation, tissue damage, and ongoing infection [43, 45, 46]
2.3.2 Isoliquiritigenin The experiment isolated the potent relaxant metabolite, isoliquiritigenin, from the aqueous extract of licorice
On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler
Two options: 2.0 mL bacteriostatic water per 5 mg vial 2.5 mg/mL (gradual), or 1.0 mL 5 mg/mL (advanced)
