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Membranes were then incubated in blocking buffer consisting of 5% non-fat skim milk and 0.02% sodium azide in Tris-buffered saline plus Tween [TBST, 10% Tween-20, 20 mM Tris-HCl (pH 7.6), and 150 mM NaCl] for 1.5 h at room temperature

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On the other hand, Lemon Bottle Vial Treatment offers a more streamlined approach, yielding quicker and more noticeable results with the advantage of minimal swelling