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An equivalent amount of protein from each animal was mixed with 4 sample buffer with or without 2-mercaptoethanol, then separated by SDSpolyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)
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Ashfaq et al., 2008
A standard Lipid Panel Test estimates the amount of cholesterol carried by LDL particles
