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Repeated freeze-thaw cycles must be avoided as they accelerate peptide bond cleavage
Some researchers add an extra 0.1mL of bacteriostatic water to compensate, accepting a slightly lower concentration (3.23mg/mL instead of 3.33mg/mL) in exchange for recovering nearly all the peptide
4Gently swirl or roll the vial until the powder is completely dissolved and the solution is clear
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