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Given the robustness of this mitochondrial response, the multifaceted nature of PGC1 regulation in cells and a modest rescue at best by mTORC1 inhibition, we suspect that there is a degree of functional redundancy built into this metabolic plasticity system that can contribute to such an adaptive response, and complete inhibition of this system will prove difficult

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Pull-down assays are typically performed in vitro using purified and labeled target proteins to capture interacting partners, making them ideal for in-depth analysis of specific protein interaction networks