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This antibody does not bind well to rat IGF1R (data not shown), so this combination of antibodies will work on some, but not all, species
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A scan cycle comprised a MS1 scan (m/z range 3351800, ion injection time of 25 ms, resolution 60,000 and automatic gain control of 3.0 106 acquired in profile mode, followed by 20 sequential dependent MS2 scans (resolution, 15,000) of the most intense ions fulfilling predefined selection criteria (AGC 5 103, maximum ion injection time 40 ms, isolation window of 1.4 m/z)
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