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This method employs a single reagent solution, simplifying the experimental procedure

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o , Application of GLP-1 and GLP-1MK-801 induced changes in fluorescence indicative of rises in intracellular calcium (%F/F 0 ) as shown by these representative fluorescent responses of two different Fura 2-AM loaded cells to application of GLP-1 (1 M) or GLP-1MK-801 (1 M)