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After expression of roGFP2 for 16 h at 20 C, as aforementioned, cells were harvested, washed in HEPES buffer (40 mM, pH 7.4) and adjusted to an OD 600 of 1.0 in HEPES buffer containing either 1 mM Aldrithriol-2 (Sigma-Aldrich, Darmstadt, Germany, CAS-2127-03-9, AT-2) (oxidation control), 10 mM Dithiothreitol (Sigma-Aldrich, Darmstadt, Germany, CAS-3483-12-3, DTT) (reduction control), or buffer

In order to facilitate robust adhesion and/or transendothelial migration, these molecules interact with counter-receptors present on leukocytes and plasminogen activator inhibitor-1 within the circulatory system, indicating the potential presence of an endothelial phenotype that is both pro-inflammatory and pro-thrombotic (90)