Introducing the sequence into skin cells enabled Thorens to establish whether it could construct the receptor (Delaye)
At least 10,000 cells per sample were analyzed in duplicates and classified into PMN and PBMC based on size and granularity by flow cytometry (FACSCantoII, BD Biosciences, San Jose, USA, Supplementary Figures 1A, D )
Since the values obtained by these equations are the amount of total glutathione in treated sample solutions, further calculations are necessary if the actual concentration of glutathione in cells or tissues needs to be determined
Given recent advances in our understanding of metformin transport in the gut, the incretin system, the potential gutbrain axis and the complexities of the microbiome, more research in these areas would doubtless further improve our knowledge and understanding of metformin action and tolerance