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The active compound turned out to be this copper-binding tripeptide
Cell membranes were solubilized by 0.5% (w/v) LMNG (Anatrace, Maumee, USA) supplemented with 0.1% (w/v) CHS (Anatrace) with 5 M GLP-2 and Protease Inhibitor Cocktail (Topscience) at 4 C for 2 h, followed by centrifugation at 65,000 g for 30 min at 4 C
Isolation of CD34 + hematopoietic stem cells from human umbilical cord blood, differentiation towards the endothelial lineage, and to brain-like endothelial cells by co-culture with PCs were previously described in detail [39]
According to the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), hypothyroidism affects approximately 5% of the US population (including both overt and subclinical cases), with higher prevalence among women and older adults
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