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Deletion of ZWF1, TRX1 and TRX2 in a BY4742 background was achieved using the primer pairs Pr7/Pr8, Pr9/Pr10, Pr11/Pr12 respectively

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Practical management based on the type of cough For reflux-related cough: Eat smaller meals, especially the evening meal Stop eating 3 hours before bed Elevate the head of the bed by 6 to 8 inches Avoid known reflux triggers (coffee, alcohol, citrus, tomato, chocolate, fatty foods) Try an OTC H2 blocker (famotidine 20 mg twice daily) for 2 to 4 weeks If no improvement, an OTC PPI (omeprazole 20 mg daily before breakfast) for 2 to 4 weeks If still no improvement, see your provider for evaluation For post-nasal drip cough: Saline nasal rinses twice daily Oral antihistamine if seasonal allergies are contributing Stay hydrated (mucus is thinner when you're well-hydrated) Humidifier at night If symptoms persist 4+ weeks, see your provider for sinusitis evaluation For dehydration-related cough: Increase water intake to 8 to 10 cups daily Add electrolytes if you're losing fluid through GI side effects Use a humidifier in dry environments Throat lozenges for symptom relief If GI side effects are severe enough to cause dehydration, contact your provider