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OTUD5 was immunopurified after an in vitro ubiquitylation reaction with TRIP12, then subjected to mass spectrometric quantification using parallel reaction monitoring (Ub-AQUA/PRM) 30

This may include obtaining appropriate informed consent from patients, ensuring that peptides are obtained from legitimate sources, and maintaining appropriate documentation of medical necessity and treatment rationale

Peroxynitrite (ONOO - ,0.1 M stock solution in 0.3 M NaOH-NaCl) was diluted to less than 1/100 with ice-cold water, just prior to quick exposure to HGF in PBS by vortexing at 1:2000 (see Figure 1a for the experimental design) 32

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