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The authors note that this relationships has been "validated across multiple disease models, including synovial macrophages in OA, bone marrow precursor cells in OP, and muscle cells in SP." Additionally, the cellular mechanisms serve an antioxidant purpose via PGC-1 activation and Nrf2 nuclear translocation, GLP-1 upregulates HO-1 and NQO1 while reducing NADPH oxidase activity and ROS generation

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04 Inject down the vial wall Insert the needle and inject the BAC water slowly down the inside wall of the KLOW vial

After tipping off the supernatant, plates were washed, blotted on paper and blocked with PBS 1% BSA for 30 min at RT