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The most common side effect at high doses (above 1,500mg IV) is transient mild hypotension or sulfur-related gastrointestinal discomfort
To construct vectors for the Y2H assays, LET7 , LET7 TPR , LET7 PPase , HOP1 , HOP1 TPR1 (600 bp, from 1 to 600), HOP1 TPR1 / 2a (1,140 bp, from 1 to 1140), HOP1 TPR2a / 2b (1,116 bp, from 601 to 1716), HOP1 TPR2b (576 bp, from 1141 to 1716) or CRCK3 SP (1,440 bp, from 91 to 1530, without the signal peptide) were amplified via PCR from the pHBT -p35S :: LET7-HA , pHBT -p35S :: HOP1-HA or pHBT -p35S :: CRCK3-HA vectors and subcloned into the pGADT7 and pGBKT7 (Clontech) vectors
Constipation is never fun, and it can be extra frustrating when its a side effect to a helpful medication
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