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Cells were imaged in fresh FluoroBrite DMEM supplemented with 4 mM glutamine, 1 mM sodium pyruvate, 10% FBS and penicillin (100 U ml 1 )/streptomycin (100 g ml 1 )/fungizone (0.25 g ml 1 )
This reduction has been linked to cognitive decline and disease progression
Collectively, these data demonstrate that NSW disrupts both the expression and methylation status of core circadian clock genes involved in BC etiology, such as PER1, PER2, BMAL1, and CLOCK (Fig
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