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All cells were cultured in a DMEM medium (Gibco, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS), 100 U/mL of penicillin, and 100 g/ml of streptomycin in 5% CO 2 at 37C

Additionally, the absorption process itself is competitive, with amino acids and other nutrients vying for uptake in the small intestine

Finally there is a substantial body of cell-culture observation on fibroblast behavior, including proliferation, migration and matrix deposition, with a smaller keratinocyte literature alongside it