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Compared with the rats treated with APAP alone, rhein treated animals demonstrated significant reduction of the biochemical indicators of liver injury, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea nitrogen (UREA), creatinine (CREA), nitric oxide (NO), and malondialdehyde (MDA)
Kinetic constants of Michaelis constant ( K m ) and maximal reaction velocity ( V max ) were determined at pH 7.6 and 25 C using the Lineweaver-Burk method [37]
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The highest fold change in the gene level - approximately 44 times higher in IL-8 expression, was observed in 580 M CuCl 2 treated cells when compared to control
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