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Although sensory ganglia are understood to be the primary source of virus establishing latency, recent studies using a modified ex vivo tissue explant reactivation assay have found that 80% of brainstem explants display viral reactivation following latent infection with KOS or McKrae HSV-1 viruses, indicating that the CNS can also be an effective source of infectious HSV-1 from which the virus reactivates (Chen et al., in vivo has also been evidenced in the brainstem before its detection in the TG, with the virus reactivating in this study more frequently from the brainstem than the TG (Yao et al., 2014)