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5-(Biotinamido)pentylamine (BP) incorporation assay Purified GST tag and human TG2 were incubated with BP (Pierce), 5 mM 1,4-dithiothreitol (DTT) (Sigma-Aldrich), and CaCl 2 (Sigma-Aldrich)
The photograph above, from Vanguard Lab, shows five of the concentrated tests showing a single visible control line on each strip, which would ordinarily be interpreted as a presumptive positive result
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