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The PCR product was digested with Sac I and Pst I and then inserted into the sites of pTrc99a digested with the same enzymes to form the plasmid pTrc99a- as
doi: 10.1016/S0304-3940(01)01618-4 191 PerryTHaugheyNJMattsonMPEganJMGreigNH
GHK-Cu explored for collagen synthesis , skin remodeling, and anti-inflammatory copper-dependent actions
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