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Nevertheless, the recanalization of acute thrombosis remains one of the most pressing clinical challenges, characterized by a highly sensitive balance between time-dependent benefits and procedural risks
NZ J Bot 6:2532
Cells or organoids were solubilized in pull-down buffer (50 mM Tris pH 7.3, 150 mM NaCl, 1% Triton X-100, 0.1 mM CaCl 2 plus protease/phosphatase inhibitors as above) and centrifuged at 14,000 rpm for 10 min at 4C
Plan to fast for a period of three to four days
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